DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae
نویسندگان
چکیده
The euryhaline copepod Pseudodiaptomus inopinus play important roles in coastal waters as vectors of docosahexanoic (DHA) and eicosapentaenoic (EPA) acids for larval fish. While DHA EPA polar lipids (PLs) are more effective fish development than non-polar lipid forms (NLs), there is little knowledge how much these accumulated copepods from microalgae early larvae. We report PLs fatty acid profiles P. fed DHA-poor evaluate its significance a food source larvae Pagrus major , compared with DHA-enriched rotifers. Copepods rotifers were mixed diet three algal species ( Phaeodactylum tricornutum Tisochrysis lutea Pavlova lutheri ), addition DHA-supplemented Super Fresh Chlorella (SFC) Compared SFC, the mixture had higher but lower DHA. total each diet. Copepod specifically enriched their contents both On other hand, comparable between preys, indicating that selectively fortified PLs. Fish culture experiment showed growth those SFC-enriched Principal component analysis organism composition emphasized trophic modification by toward This study highlighted contribute to enhanced efficiently transferring via metabolism.
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Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, Pagrus major, using glycolchitin and N-acetylchitooligosaccharides (GlcNAc(n), n=2-6) as substrates. High activity was observed at two pHs, such as 2.5 and 9.0, toward glycolchitin as seen in other insect chitinases, and also at both pH 2.5 and 5.0 even toward a short substrate, N-acetylc...
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ژورنال
عنوان ژورنال: Frontiers in Marine Science
سال: 2021
ISSN: ['2296-7745']
DOI: https://doi.org/10.3389/fmars.2021.632876